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Effects of different DG molecular species on PKCα activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) <t>and</t> <t>PKC</t> activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in .The results are the means±SD of four independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05).

βιι, supplied by Carna Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species"

Article Title: Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species

Journal: Biochemistry and Biophysics Reports

doi: 10.1016/j.bbrep.2016.07.017

Figure Legend Snippet: Effects of different DG molecular species on PKCα activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in .The results are the means±SD of four independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05).

Techniques Used: Activation Assay, Activity Assay

Effects of different DG molecular species on PKCβΙΙ activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means ± SD of three independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005).

Figure Legend Snippet: Effects of different DG molecular species on PKCβΙΙ activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means ± SD of three independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005).

Techniques Used: Activation Assay, Activity Assay

Effects of different DG molecular species on PKCγ activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means±SD of five independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05).

Figure Legend Snippet: Effects of different DG molecular species on PKCγ activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means±SD of five independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05).

Techniques Used: Activation Assay, Activity Assay

Effects of different DG molecular species on PKCδ activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means±SD of four independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05, ## P <0.01).

Figure Legend Snippet: Effects of different DG molecular species on PKCδ activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means±SD of four independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05, ## P <0.01).

Techniques Used: Activation Assay, Activity Assay

Effects of different DG molecular species on PKCε activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means±SD of four independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05).

Figure Legend Snippet: Effects of different DG molecular species on PKCε activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means±SD of four independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05).

Techniques Used: Activation Assay, Activity Assay

Effects of different DG molecular species on PKCη activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means±SD of four independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity.

Figure Legend Snippet: Effects of different DG molecular species on PKCη activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means±SD of four independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity.

Techniques Used: Activation Assay, Activity Assay

Effects of different DG molecular species on the activation of PKCη expressed in COS-7 cells. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The activities of vector-transfected cells were subtracted. The results are the means±SD of three independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG). The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05, ## P <0.01).

Figure Legend Snippet: Effects of different DG molecular species on the activation of PKCη expressed in COS-7 cells. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The activities of vector-transfected cells were subtracted. The results are the means±SD of three independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG). The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05, ## P <0.01).

Techniques Used: Activation Assay, Activity Assay, Plasmid Preparation, Transfection

Effects of different DG molecular species on PKCθ activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs as described in . The results are the means ± SD of three independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05, ## P <0.01).

Figure Legend Snippet: Effects of different DG molecular species on PKCθ activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs as described in . The results are the means ± SD of three independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05, ## P <0.01).

Techniques Used: Activation Assay, Activity Assay

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Journal: Biochemistry and Biophysics Reports

Article Title: Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species

doi: 10.1016/j.bbrep.2016.07.017

Figure Lengend Snippet: Effects of different DG molecular species on PKCα activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in .The results are the means±SD of four independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05).

Article Snippet: PKC isoforms (α (product number: 01-133), βΙΙ (01-165), γ (01-137), δ (01-135), ε (01-136), η (01-138) and θ (01-140)) were obtained from Carna Biosciences (Kobe, Japan).

Techniques: Activation Assay, Activity Assay

Journal: Biochemistry and Biophysics Reports

Article Title: Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species

doi: 10.1016/j.bbrep.2016.07.017

Figure Lengend Snippet: Effects of different DG molecular species on PKCβΙΙ activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means ± SD of three independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005).

Techniques: Activation Assay, Activity Assay

Journal: Biochemistry and Biophysics Reports

Article Title: Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species

doi: 10.1016/j.bbrep.2016.07.017

Figure Lengend Snippet: Effects of different DG molecular species on PKCγ activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means±SD of five independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05).

Techniques: Activation Assay, Activity Assay

Journal: Biochemistry and Biophysics Reports

Article Title: Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species

doi: 10.1016/j.bbrep.2016.07.017

Figure Lengend Snippet: Effects of different DG molecular species on PKCδ activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means±SD of four independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05, ## P <0.01).

Techniques: Activation Assay, Activity Assay

Journal: Biochemistry and Biophysics Reports

Article Title: Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species

doi: 10.1016/j.bbrep.2016.07.017

Figure Lengend Snippet: Effects of different DG molecular species on PKCε activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means±SD of four independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05).

Techniques: Activation Assay, Activity Assay

Journal: Biochemistry and Biophysics Reports

Article Title: Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species

doi: 10.1016/j.bbrep.2016.07.017

Figure Lengend Snippet: Effects of different DG molecular species on PKCη activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The results are the means±SD of four independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity.

Techniques: Activation Assay, Activity Assay

Journal: Biochemistry and Biophysics Reports

Article Title: Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species

doi: 10.1016/j.bbrep.2016.07.017

Figure Lengend Snippet: Effects of different DG molecular species on the activation of PKCη expressed in COS-7 cells. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs, as described in . The activities of vector-transfected cells were subtracted. The results are the means±SD of three independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG). The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05, ## P <0.01).

Techniques: Activation Assay, Activity Assay, Plasmid Preparation, Transfection

Journal: Biochemistry and Biophysics Reports

Article Title: Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species

doi: 10.1016/j.bbrep.2016.07.017

Figure Lengend Snippet: Effects of different DG molecular species on PKCθ activation. Lipid vesicles were prepared with different DG molecular species (16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- and 18:0/22:6-DG) and PKC activity toward MBP was determined in vesicles as a function of increasing concentrations of DGs as described in . The results are the means ± SD of three independent experiments. The left axis shows the relative activity compared to the control (0 mmol% DG) and the right axis shows the specific activity. The data are significantly different from the control, 0 mmol% DG (* P <0.05, ** P <0.01, *** P <0.005), and among the DG molecular species ( # P <0.05, ## P <0.01).

Techniques: Activation Assay, Activity Assay

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